Yeast RGB

Screen Selection

Available Screens

Selected Screens

Name	Original Collection	Genotype	Number of Proteins	Strain details	Screen pictures (TIFF)	Reference
C' GFP	"Yeast GFP Clone"	BY4741 background ORF::GFP-HIS3	6234	TSV	NORM (ZIP 5.3GB) RAW (ZIP 5.8GB)	[1] [2]
N' SWAT GFP	N-terminal "Yeast SWAp-Tag" (SWAT)	BY4741 + Y8205 background ORFpromoter::-L1-mCherry-spNOP1-sfGFP-L2::ORF	5569	TSV	NORM (ZIP 5.9GB) RAW (ZIP 5.9GB)	[3] [4]
C' SWAT GFP	C-terminal "Yeast SWAp-Tag" (SWAT)	BY4741 + Y8205 background ORF:: L3-mNeonGreen-hph-ALG9term-L4 crossed with BY4741 background NUP49::mCherry-natNT2	5661	TSV	NORM (ZIP 8.3GB) RAW (ZIP 9.1GB)	[5] [6]

[1] : Ghaemmaghami S, Huh WK, Bower K, et al., Global analysis of protein expression in yeast. Nature. 2003;425(6959):737-41
[2] : Huh WK, Falvo JV, Gerke LC, et al., Global analysis of protein localization in budding yeast. Nature. 2003;425(6959):686-91
[3] : Yofe I, Weill U, Meurer M, et al., One library to make them all: Streamlining yeast library creation by a SWAp-Tag (SWAT) strategy. Nature Methods. 2016;13(4):371-378.
[4] : Weill U, Yofe I, Sass E, et al., Genome-wide SWAp-Tag yeast libraries for proteome exploration. Nature Methods 2018;15(8):617-622
[5] : Meurer M, Duan Y, Sass E, et al., Genome-wide C-SWAT library for high-throughput yeast genome tagging. Nature Methods 2018;15(8):598-600
[6] : Meurer M, Duan Y, Sass E, et al., A genome-wide resource for high-throughput genomic tagging of yeast ORFs. BioRxiv 2017;226811

YeastRGB is hosted in the laboratory of Emmanuel Levy at the Weizmann Institute of Science .

The N’ and C’ tagged SWAT yeast collections were created in the laboratories of Maya Schuldiner and Michael Knop.

Imaging of strains was performed using a confocal spinning disk microscope (Yokogawa W1) with a 60x oil objective. Several people contributed to imaging the collections:

Joseph Georgeson (MSc)
Meta Heidenreich (PhD)
Yotam Nadav (Technician)
Ehud Sass (Dr)

Changes history

YeastRGB v1.0
- Design (HTML and CSS)
  - 'About' window design, text and animation
  - Tutorial slideshow animation and slides creation
  - Screen selection dropdown panel
  - Adding 'loading' overlay while creating gallery of display units
  - Optimization of display units content shown (Collapsible, statistics table, decrease line height...)
- Coding (JS and Jquery)
  - Live search results show in an HTML table
  - JS function to create display unit
  - Download feature added
  - Optimizing adjustable gallery layout
YeastRGB v1.1
- Paginated gallery for more than 10 proteins
- Highlight matched query in search table
- Fix design bugs (Description and Localization overflow display units in 'column' gallery layout)
- Page status object to keept track of user navigation preferences (Layout, default channel & zoom)
- Pressing "Enter" key triggers search and loads the gallery
- Highlight matched query in search table
- Restructuring JS flow:
  - 1. Filter table with matched rows
  - 2. Initialize page status
  - 3. Prepare pagination of results
  - 4. Preload images and data
  - 5. Prepare gallery
  - 6. Load display units of first ten results in page #1
YeastRGB v1.2
- Correct table columns width and set description overflowing text display on mouse hover
- Added columns to indicate whether GO CC terms are matched
- Added genotype of the yeast strains and the number of strains present for each screen
- Button to hide Statistics and Infos section in display unit (Ultra Compact)
YeastRGB v1.3
- Added instructions for uploading data to yeastRGB
- Added links to raw images (without normalization of pixel intensities)

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